Salmonella Research Basque Group
Home:
University of the Basque Country - www.ehu.es
Dept. Inmunol., Microbiol. y Parasitol.
Paseo de la Universidad 7
01006 Vitoria-Gasteiz, Spain
Tel: 34 945 013912 - Fax 34 945 013014
Principal investigator:
Dr. Javier Garaizar, M.D.
email: javier.garaizar at ehu.es
Research team:
Aitor Rementeria aitor.rementeria at ehu.es
Joseba Bikandi joseba.bikandi at ehu.es
Ana Vivanco
Ilargi Martínez
Carolina Muguruza
Lorena Laorden
Ana Bernal
Fields of interest:
Molecular epidemiology of Salmonella
Genomics and bioinformatics of Salmonella
Molecular diagnostic procedures of Salmonella in clinical and food samples
Curriculum:
After passing a postdoctoral stage at the Laboratory of Hospital Infection, CPHL-London for a year, I started the research in epidemiology of infectious diseases and the use of molecular epidemiological markers for outbreak control. The combinations of phenotypical and genotypical microbial makers are extremely useful for the detection of outbreaks of infectious agents. In 1994 the Salmonella research laboratory started its activity at the Faculty of Pharmacy, University of the Basque Country in Vitoria-Gasteiz, Spain. From that time, we had had cooperative projects with colleagues from our own country and from Denmark, The Netherlands, United Kingdom and the United States of America. The techniques used at the projects were ribotyping, REA, PFGE, RAPD and PCR. Recently, we open our research to genomics, bioinformatics and the molecular diagnosis of Salmonella, using RNA and DNA detection, hybridization, microarrays, Real Time PCR, fluorescent probes, and bioinformatic tools (http://insilico.ehu.es/). I belong to the Epidemiological Markers Study Group at the European Society of Clinical Microbiology and Infectious Diseases, The Spanish Society of Clinical Microbiology and Infectious Diseases, The Spanish Society for Microbiology, and I am member of the Editorial board of the journal Molecular and Cellular Probes. As we are a university based research laboratory our focus is science and student skills development. Besides of an intensive pregraduate teaching, we run a postgraduate course in Epidemiology of Infectious Diseases, direct several Ph.D. Thesis, and receive Erasmus students from abroad. We are interested in collaborative research projects with academic or other institutions of the Mediterranean area in Salmonella subject and other enteropathogens.
Key publications:
R. CISTERNA, A. UMARAN, E. SUÑEN, J. GARAIZAR, M. ARETXEDERRA, A. FDEZ DE ARANGUIZ. 1989 Activity of ciprofloxacin and ofloxacin and four beta-lactam agents against hospital bacterias strains. Reviews of Infectious Diseases. 11;sup.5: 1143-1144.
L. GALLEGO, A. UMARAN, J. GARAIZAR, K COLOM, R. CISTERNA. 1990 Digoxigenin-labeled DNA probe to detect TEM type betalactamase. Journal of Microbiological Methods. 11: 261-267. Scopus
J. GARAIZAR, M. E. KAUFMANN, T. L. PITT. 1991 Comparison of ribotyping with conventional methods for the type identification of Enterobacter cloacae. Journal of Clinical Microbiology. 29: 1303-1307. PubMed
A. FDEZ. DE ARANGUIZ, R. ALONSO, K. COLOM, L. GALLEGO, A. UMARAN, J. GARAIZAR, R. CISTERNA 1992 Resistencia antimicrobiana en cepas de Salmonella enterica aisladas en el Hospital de Basurto (Bilbao) desde 1987 hasta 1990. Enfermedades Infecciosas y Microbiología Clínica. 2:89-96. PubMed
L. DIJKSHOORN, H. AUCKEN, P. GERNER-SMIDT, P. JANSSEN, M. E. KAUFMANN, J. GARAIZAR, J. URSING, T. L. PITT. 1996 Comparison of outbreak and non-outbreak Acinetobacter baumanii strains by genotypic and phenotypic methods. Journal of Clinical Microbiology. 34:1519-1525. PubMed
M. J. STRUELENS, P. GERNER-SMIDT, V. ROSDAHL, N .EL-SOHL, J. ETIENNE, M. H. NICOLAS, U. RÖMLING, W. WITTE, N. LEGAKIS, A. VAN BELKUM, L. DIJKSHOON, H. LENCASTRE, J. GARAIZAR, D. BLANC, A. BAUERNFEIND, B. D. COOKSON,TL. PITT. 1996 Guidelines for appropriate use and evaluation of microbial epidemiological typing systems. Clinical Microbiology and Infection. 2:2-11. Scopus
I. LACONCHA, N. LÓPEZ-MOLINA, A. REMENTERÍA, A. AUDICANA, I. PERALES, J. GARAIZAR. 1998 Phage typing combined with pulsed-field gel electrophoresis and random amplified polymorphic DNA increases discrimination in the epidemiological analysis of Salmonella enteritidis strains. International Journal of Food Microbiology. 40;27-34. PubMed
N. LÓPEZ-MOLINA, I. LACONCHA, A. REMENTERÍA, A. AUDICANA, I. PERALES, J. GARAIZAR. 1998 Typing of Salmonella serotypes including Salmonella enteritidis of different phage types by PCR fingerprinting. Journal of Applied Microbiology. 84:877-882. PubMed
I. LACONCHA, D. L. BAGGESEN, A. REMENTERIA, J. GARAIZAR. 2000 Genotypic characterisation by PFGE of Salmonella enterica serotype Enteritidis phage types 1, 4, 6, and 8 isolated from animal and human sources in three European countries. Veterinary Microbiology. 75: 155-165. PubMed
J. GARAIZAR, N. LOPEZ-MOLINA, I. LACONCHA, D. L. BAGGESEN, A. REMENTERIA, A, VIVANCO, A. AUDICANA, I. PERALES. 2000 Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerised gel analysis, in library typing of Salmonella enteritidis. Applied and Environmental Microbiology. 66:5273-5281. PubMed
A. REMENTERIA, L. GALLEGO, G. QUINDOS, J. GARAIZAR. 2001. Comparative evaluation of three commercial software packages for analysis of DNA polymorphism patterns. Clinical Microbiology and Infection. 7:331-336. PubMed
M. A. ECHEITA, S. HERRERA., J. GARAIZAR, M. A. USERA. 2002 Multiplex PCR-based detection and identification of the most common Salmonella second-phase flagellar antigens. Research in Microbiology. 153:107-113. PubMed
J. GARAIZAR, S. PORWOLLIK, M. A ECHEITA, A. REMENTERIA, S. HERRERA, R. M. Y. WONG, J. FRYE., M. A. USERA, M. McCLELLAND. 2002. DNA microarray-based typing of atypical monophasic Salmonella serovar [4,5,12:i:-] strains emergent in Spain. Journal of Clinical Microbiology. 40:2074-2078. PubMed
S. MURCHAN, M.E. KAUFMANN, A. DEPLANO, R. DE RYCK, M. STRUELENS, C. ELSBERG-ZINN, V. FUSSING,S. SALMELINNA, J. VUOPIO-VARKILA, E. EL-SOLH, C. CUNY, W. WITTE, P. T. TASSIOS, N. J. LEGAKIS, W. VAN-LEEUWEN, A. VAN-BELKUM, A. VINDEL, I. LACONCHA, J. GARAIZAR, S. HAEGGMAN, U. RANSJO, B. OLSSON-LILJEQUIST, G. COOMBES, B. D. COOKSON. 2003 Harmonisation of Pulsed-Field Gel Electrophoresis for epidemiological typing of methicillin-resistant Staphylococcus aureus by consensus in 10 European centres and its application for tracing the spread of related strains. Journal of Clinical Microbiology. 41:1574-85. PubMed
J. ALVAREZ, S. PORWOLLIK, I. LACONCHA, V. GISAKIS, A. B. VIVANCO, I. GONZALEZ, S. ECHENAGUSIA, N. ZABALA, F. BLACKMER, M. MCCLELLAND, J. GARAIZAR. 2003 Detection of a Salmonella enterica serovar California strain spreading in Spanish feedmills and its genetic characterization using DNA microarray. Applied and Environmental Microbiology. 69:7531-7534. PubMed
A. REMENTERIA, A. B. VIVANCO, A. CADAVAL, M. T. RUESGA, S. BRENA, J. PONTON, G. QUINDOS, and J. GARAIZAR. 2004 Molecular methods and computerized analysis for typing fungal isolates. pp: 117-126, En: Public Health Microbiology: methods and protocols. (Spencer J. F. T., Ragout de Spencer A. L. Eds.) Methods in Molecular Biology 268. Humana Press. Totowa, New Jersey, EEUU. E-ISBN 1-59259-766-1. PubMed
A. VIVANCO, J, ALVAREZ, I. LACONCHA, N. LOPEZ-MOLINA, A. REMENTERIA and J. GARAIZAR. 2004 Molecular genotyping methods and computerized analysis for the study of Salmonella enterica. pp:49-58, En: Public Health Microbiology: methods and protocols. (Spencer J. F. T., Ragout de Spencer A. L. Eds.) Methods in Molecular Biology 268. Humana Press. Totowa, New Jersey, EEUU. E-ISBN 1-59259-766-1. PubMed
J. BIKANDI, R. SAN MILLAN, A. REMENTERIA, J. GARAIZAR. 2004 In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction. Bioinformatics 20:789-9. PubMed
J. ALVAREZ, M. SOTA, A. B. VIVANCO, I. PERALES, R. CISTERNA, A. REMENTERIA, J. GARAIZAR. 2004 Development of a multiplex-PCR technique for Salmonella detection and epidemiological typing in human clinical samples. Journal of Clinical Microbiology. 42:1734-8. PubMed
S. HERRERA-LEON, J. R. McQUISTON, M. A. USERA, P. I. FIELDS, J. GARAIZAR, M. A. ECHEITA. 2004. Multiplex PCR for distinguishing the most common phase-1 flagellar antigens of Salmonella spp. Journal of Clinical Microbiology 42:2581-6. PubMed
A. REMENTERIA, N. LOPEZ-MOLINA, A. LUDWIG, A. B. VIVANCO, J. BIKANDI, J. PONTON, J. GARAIZAR. 2005 Genes and molecules implicated in the virulence of Aspergillus fumigatus. Revista Iberoamericana de Micología. 22: 1-23. PubMed
R. ALONSO, E. MATEO, M. GALIMAND, J. GARAIZAR, P. COURVALIN, R. CISTERNA. 2005 Clonal spread of pediatric isolates of ciprofloxacin-resistant, emm Type 6 Streptococcus pyogenes. Journal of Clinical Microbiology. 43:2492-2493. PubMed
R. SAN MILLAN, J. GARAIZAR, J. BIKANDI. 2005 In silico simulation of fingerprinting techniques based on double endonuclease digestion of genomic DNA. In Silico Biology 5:1-5. PubMed
J. GARAIZAR, A. REMENTERIA, S. PORWOLLIK. 2006 DNA microarray technology: a new tool for the epidemiological typing of bacterial pathogens?. FEMS Immunology and Medical Microbiology. 47:178-189. PubMed
F. LOPITZ-OTSOA, A. REMENTERIA, N. ELGUEZABAL, J. GARAIZAR 2006. Kefir: a symbiotic yeast-bacteria community with alleged healthy capabilities. Revista Iberoamericana de Micología. 23:67-74. PubMed
J. GARAIZAR, S. BRENA, J. BIKANDI, A. REMENTERIA, J. PONTON. 2006 Use of DNA microarray technology and gene expression profiles at the pathogenesis, cell biology, antifungal susceptibility, and diagnosis of Candida albicans. FEMS Yeast research. 6:987-998. PubMed